Association between ABCA1 DNA methylation levels and acetylsalicylic acid therapy. Subjects on acetylsalicylic acid (ASA) therapy had lower mean ATP-binding cassette transporter A1 (ABCA1) DNA methylation levels (Δ = −3.6%) compared to subjects not on ASA therapy, independent of age and CAD. In subjects without CAD, we observed the same significant association between ASA and a lower mean ABCA1 DNA methylation level (Δ = −4.7%; P = 0.01). Although not significant, we observed that in subjects with CAD, those under an ASA treatment exhibited a similar decrease in mean ABCA1 DNA methylation levels (Δ = −3.9%; P = 0.14). aP-values were obtained after consideration of age. bP-value was obtained after consideration of age and CAD status.