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Figure 4 | Clinical Epigenetics

Figure 4

From: Epigenetic response in mice mastitis: Role of histone H3 acetylation and microRNA(s) in the regulation of host inflammatory gene expression during Staphylococcus aureus infection

Figure 4

S. aureus infection specifically induces histone H3K9 and H3K14 - acetylation in mice. (A) SA1 and SA2 inoculated mouse mammary tissues were analyzed by western blots using antibodies specific to acetylated H3K9 (I), H3K14 (II), H4K8 (III), and H4K12 (IV), methylated H3K36 (V), and phosphorylated H3S10 (VI). The levels of histone H3 (VII) and β-actin (VIII) were used as loading controls. Lane 1, PBS inoculated, Lane 2, SA1 inoculated and Lane 3, SA2 inoculated mouse mammary tissue. (B) Densitometric analysis of western blots. Western blots were scanned by Bio-Rad Geldoc XR scanner and band intensities were quantified by Quantity One™ software. Each lane was independently compared with PBS control after normalization with H3. The box-plots show median band intensities of three independent western blots.

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