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Table 3 Primers used for promoter CpG analysis

From: Differential placental methylation and expression of VEGF, FLT- 1 and KDR genes in human term and preterm preeclampsia

Primer code

Primer sequence (5′-3′)*

Amplicon length (base pairs)

Number of CpG sites analyzed

VEGF_F1

aggaagagagTTAGAAATAGGGGGTTTAGGAGTAAAT

465

23

VEGF_R1

cagtaatacgactcactatagggagaaggctCCAAATTCACAACCTAAAAATTACCCA

FLT1_F1

aggaagagagGTTTTTAGGAAGTAGAAGATTGAGG

444

30

FLT1_R1

cagtaatacgactcactatagggagaaggctCTCAAAAAATCCTTACAAACTCCCT

KDR_F1

aggaagagagTTTTAGAGTGGGTTTTTTATTTATAGAGG

403

37

KDR_R1

cagtaatacgactcactatagggagaaggctAACTCCCACCCTACACTAAATCC

  1. * T7-promoter sequence tag -cagtaatacgactcactatagggagaaggct added to reverse primer.
  2. 10-mer sequence tag- aggaagagag added to the forward primer.
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Clinical Epigenetics

ISSN: 1868-7083

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