Skip to main content

Table 3 Primers used for promoter CpG analysis

From: Differential placental methylation and expression of VEGF, FLT- 1 and KDR genes in human term and preterm preeclampsia

Primer code Primer sequence (5′-3′)* Amplicon length (base pairs) Number of CpG sites analyzed
VEGF_F1 aggaagagagTTAGAAATAGGGGGTTTAGGAGTAAAT 465 23
VEGF_R1 cagtaatacgactcactatagggagaaggctCCAAATTCACAACCTAAAAATTACCCA
FLT1_F1 aggaagagagGTTTTTAGGAAGTAGAAGATTGAGG 444 30
FLT1_R1 cagtaatacgactcactatagggagaaggctCTCAAAAAATCCTTACAAACTCCCT
KDR_F1 aggaagagagTTTTAGAGTGGGTTTTTTATTTATAGAGG 403 37
KDR_R1 cagtaatacgactcactatagggagaaggctAACTCCCACCCTACACTAAATCC
  1. * T7-promoter sequence tag -cagtaatacgactcactatagggagaaggct added to reverse primer.
  2. 10-mer sequence tag- aggaagagag added to the forward primer.