Azacitidine treatment reduces the anchorage independent growth of renal cell carcinoma (RCC) cell lines. (A) Pre-treatment with 3 μM azacitidine resulted in reduction of anchorage-independent growth in all seven RCC cell lines tested. Other cell lines did not grow suspended in agar. Error bars represent standard error. (B) Representative images (6 weeks after seeding in agar) of control colonies and colonies formed after azacitidine pre-treatment. (C) Illumina GoldenGate Methylation Cancer Panel I Beadarray cluster analysis results of RCC cell lines. The four cell lines, SKRC18, A704, 796-P and A498 that demonstrated a 50% or greater colony reduction at both 1 μM and 3 μM azacitidine doses are highlighted with grey boxes. The three cell lines with the greatest (>50%) reduction in anchorage-independent colony formation, SKRC45, CAKI-1 and 769-P, are highlighted with red boxes.