Figure 5
From: Transcription factor NFκB regulates the expression of the histone deacetylase SIRT1
Binding of NFκB to DNA fragments of truncated SIRT1 promoter. U937 cells were treated with TNFα to induce NFκB expression. Nuclear extracts of TNFα-treated and untreated control cells were incubated with different biotin labeled DNA fragments of the SIRT1 promoter and were analyzed by electrophoretic mobility shift assay. An NFκΒ oligonucleotide was used as the positive control.