Figure 3
From: Transcription factor NFκB regulates the expression of the histone deacetylase SIRT1
Histone acetylation status in response to SIRT1 activity. Cells were transfected with expression vectors for different NFκB subunits. Expression vector for SIRT1 was used as a positive control. Histones were isolated after 24 hours and acetylation status was assessed by western blot. Transfection with empty vector was used as a reference control. ( A ) Detection was carried out with an antibody specific for acetylated histone H4K16 and with another antibody binding to histone H4 independently of the acetylation status (total H4). ( B ) Detection films of blots were scanned and quantified in silico. Values of H4K16 were normalized to total H4 and fold changes relative to control were calculated. n = 3. *P >0.05 versus empty vector.