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Table 2 Relationships between p14 ARF promoter methylation and clinicopathological data, p53 and MDM2 protein expression, TP53 mutational status, and microsatellite instability phenotype

From: Hypermethylation of the 5′ CpG island of the p14ARF flanking exon 1β in human colorectal cancer displaying a restricted pattern of p53 overexpression concomitant with increased MDM2 expression

Clinicopathological and molecular parameters Overall (%) p14 ARF promoter methylation profile
Unmethylated Dense or partial methylation P value
Age, years, mean ± SD   64 ± 12 69 ± 10 0.20571
Gender  
 Male 16 (46%) 5 (45.5%) 11 (45.8%) 0.9833
 Female 19 (54%) 6 (54.5%) 13 (54.2%)
Type of tissue     
 Tumor 35 11 (31%) 24 (69%) 0.0019
 Adjacent colon mucosa 35 24 (69%) 11 (31%)
Tumor location
 Right side 10 (29%) 3 (27.3%) 7 (29.2%) >0.05
 Left side 25 (71%) 8 (72.7%) 17 (70.8%)  
Differentiation  
 Well or moderate 22 (62.9%) 10 (90.9%) 12 (50%) 0.0270
 Poor or mucinous 13 (37.1%) 1 (9.1%) 12 (50%)  
Clinical stage  
 Stage I 3 (8.6%) - 3 (12.5%) 0.46742
 Stage II 17 (48.6%) 5 (45.4%) 12 (50%)  
 Stage III 9 (25.7%) 3 (27.3%) 6 (25%)  
 Stage IV 6 (17.1%) 3 (27.3%) 3 (12.5%)  
p53 immunohistochemistry  
 Negative pattern 5 (14.3%) 3 (27.3%) 2 (8.3%) 0.02752
 Diffuse pattern 13 (37.1%) 6 (54.5%) 7 (29.2%)  
 Restricted overexpression 17 (48.6%) 2 (18.2%) 15 (62.5%)  
MDM2 immunohistochemistry  
 Negative 12 (34.3%) 7 (63.6%) 5 (20.8%) 0.0223
 Overexpression 23 (65.7%) 4 (36.4%) 19 (79.2%)  
p21 immunohistochemistry  
 Loss to mild 9 (25.7%) 6 (54.5) 3 (12.5%) 0.01462
 Moderate to high 26 (74.3%) 5 (45.5) 21 (87.5%)  
TP53 mutational status  
 Mutation present 10 (28.6%) 5 (45.5%) 5 (20.8%) 0.2266
 No mutation detected 25 (71.4%) 6 (54.5%) 19 (79.2%)  
MSI status  
 MSI-H 11 (31.4%) 1 (9.1%) 10 (41.7%) 0.0539
 MSS 24 (68.6%) 10 (90.9%) 14 (58.3%)  
  1. Abbreviations: MSI, Microsatellite instability, MSI-H, microsatellite instability-high; MSS, microsatellite-stable.
  2. 2Two-sided two-sample t-test.
  3. 2Two-tailed Fisher’s exact test.