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Figure 1 | Clinical Epigenetics

Figure 1

From: Hypermethylation of the 5′ CpG island of the p14ARF flanking exon 1β in human colorectal cancer displaying a restricted pattern of p53 overexpression concomitant with increased MDM2 expression

Figure 1

Methylation of the p14ARFpromoter in tumors and normal colon mucosa from patients with colorectal cancer (A) p14ARFpromoter methylation analysis by methylation-specific PCR revealed coexistence of both unmethylated (U) and methylated (M) PCR products in tumor (T) and adjacent colon mucosa (N). (B) Extensive methylation of p14ARF promoter in tumors. The methylated PCR product was predominantly detected in tumor, whereas the adjacent colon mucosa produced both the unmethylated and methylated PCR products. MW, standard molecular weight, control +, positive control for methylated allele, (bisulfite-modified genomic blood DNA pretreated with the CpG methylase (M.SssI)); H2O, negative control with water only.

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