Skip to main content

Advertisement

Table 3 Details of PCR primers used for molecular genetic studies

From: Putative molecular mechanism underlying sperm chromatin remodelling is regulated by reproductive hormones

Rat genes PCR product (bp) PCR primers Gene ID/Accession /GenBank number
ube1x   Left primer: 5-GAG-AGG-AAA-TGG-TTC-TCA-CAG-ATT-3  
363 Right primer: 5-AAT-ACT-TGG-CAA-AGT-CAG-TCA-TCA-3 314432
E217Kb/   Left primer: 5-CAC-AGT-GGT-CTC-CAG-CAC-TAA-CTA-3  
ube2d2 379 Right primer: 5-GCT-AGG-AGA-CAG-TGT-TGG-TAC-AGA-3 U56407
huwe1 mouse fragment (ure-b1/e3)   Left primer: 5-GGA-GTA-TGT-GAA-GTT-CGT-GAC-CTC-3  
365 Right primer: 5-GTA-CCT-GTG-ACA-AAC-TGG-AGG-AAC-3 59026
hdac6   Left primer: 5-ACA-ACT-GCA-CAG-TGT-GCT-TCAG-3  
417 Right primer: 5-ACA-AGG-TTG-GGA-CAC-ATC-TAGG-3 84581
ubB   Left primer: 5-GTT-TGT-TCC-TTC-ATC-GCA-TTC-3  
238 Right primer: 5-GTG-CAG-GGT-TGA-CTC-TTT-CTG-3 192255
cdyl   Left primer: 5-CTA-CCT-AAG-AGC-ACT-CAC-CTG-AAG-3  
344 Right primer: 5-ACA-CTT-GAT-CGG-GAT-CTG-AGAC-3 361237
brdt   Left primer: 5-CTC-AAG-CTG-CCT-GAC-TAT-TAC-ACC-3  
382 Right primer: 5-GAG-AGA-GAC-AGA-CAT-CAG-GAA-ACC-3 XM_573544
hdac1   Left primer: 5-GAT-GTT-TCA-GCC-TAG-TGC-AGTG-3  
162 Right primer: 5-GTA-TAG-CCA-TCT-CCT-CCC-AACA-3 AF321129
miwiI/   Left primer: 5-GAG-AGG-TTA-CAA-CCC-AAG-ACT-GAC-3  
piwiLi1 400 Right primer: 5-GAG-GTA-GTA-GAG-ACG-GTT-GGA-CAG-3 363912
mbd2   Left primer: 5-GGC-AAG-AGC-GAT-GTC-TAC-TAC-TTC-3  
357 Right primer: 5-GCT-AAG-TCC-TTG-TAG-CCT-CTT-CTC-3 680172
pem(rhoX)   Left primer: 5-GAG-TGT-CAA-GTC-TGA-GGA-TAA-GCA-3  
187 Right primer:5-GTA-TGC-AGC-CCT-CCT-AAT-CTT-AAA-3 NM_022175
cremΔC-G   Left primer: 5-AGT-CTG-TAC-AGT-CCC-CAG-CAA-CTA-3  
variant 322 Right primer: 5-GCA-AGG-GTT-AAG-AGA-CCC-ATC-TAC-3 U048351
h2a somatic   Left primer: 5-GAA-TAC-CTG-ACT-GCT-GAG-ATC-CTG-3  
320 Right primer: 5-ACA-GGC-TCA-GTG-TAC-AGC-ACT-TC-3 64646
h2b somatic   Left primer: 5-AGA-AGA-AGG-ACG-GCA-AGG-AAC-3  
113 Right primer: 5-CAT-GGC-CTT-GGA-AGA-GAT-TC-3 64647
h3 somatic   Left primer: 5-TCT-CAT-GAT-GCA-TGT-TTC-TGT-ATG-3  
345 Right primer: 5-CAC-ACA-CCT-TAA-TGA-CAA-GAC-TCC-3 117056
th2a   Left primer: 5-CTC-TTT-CAG-AGC-AGG-TTT-GCAG-3  
320 Right primer: 5-ACT-TGG-TCT-GGG-ACT-TGT-GGT-3 24828
th2b   Left primer: 5-AGT-CAC-CAA-GAC-CCA-GAA-GAAG-3  
113 Right primer: 5-CTC-GAA-GAT-GTC-TGT-CAC-GAAG-3 24829
h3t   Left primer: 5-ACG-TGA-AAT-CGC-TCA-GGA-CT-3  
196 Right primer: 5-TTA-TGC-TCT-CTC-CCC-TCG-AA-3 682330
h4   Left primer: 5-AGG-AGC-CCA-GAG-TTT-GTT-ACAC-3  
399 Right primer : 5-CTG-GGA-GCA-ATC-TAG-AAG-GATG-3 64627
Mouse β actin   Left primer: 5-CTG-GCA-CCA-CAC-CTT-CTA-3  
238 Right primer: 5-GGG-CAC-AGT-GTG-GGT-GAC-3 007393(44)