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Table 1 Expression of chromatin condensing genes in FD/CPA-treated rat testis

From: Putative molecular mechanism underlying sperm chromatin remodelling is regulated by reproductive hormones

Rat genes PCR product (bp) Ratio
(Mean ± S.D.) = IOD of specific gene/ β Actin
Control FD CPA
ube1) 363 0.271 ± 0.03 0.124 ± 0.06 0.079 ± 0.02a
ube2d2 379 1.261 ± 0.016 1.324 ± 0.052 1.564 ± 0.07a
huwe1(e3) 365 0.41 ± 0.087 0.35 ± 0.04 0.33 ± 0.16
hdac6 417 0.13 ± 0.003 0.108 ± 0.01 0.061 ± 0.001a
ubB 238 -(monoplex) N N
cdyl 344 0.824 ± 0.02 0.246 ± 0.009a 0.602 ± 0.002a
brdt 382 1.826 ± 0.03 1.43 ± 0.018a 1.40 ± 0.02a
hdac1 162 0.668 ± 0.062 0.53 ± 0.13 0.41 ± 0.05a
miwi/piwi 400 1.115 ± 0.003 0.92 ± 0.25 0.925 ± 0.02a
mbd2 357 0.13 ± 0.01 0.109 ± 0.007 0.073 ± 0.01a
pem 187 0.0154 ± 0.0006 0.02 ± 0.003 0.0015 ± 0.0003a
cremΔC-G 322 -(monoplex) N N
h2a somatic 320 0.131 ± 0.01 0.17 ± 0.0009 0.05 ± 0.005a
h2b somatic 113 0.094 ± 0.005 0.099 ± 0.003 0.059 ± 0.007a
h3 somatic 345 0.549 ± 0.07 0.37 ± 0.03 0.47 ± 0.18
th2a 320 0.872 ± 0.053 0.877 ± 0.06 0.712 ± 0.002
th2b 113 3.4426 ± 0.66 3.11 ± 0.73 3.25 ± 0.217
h3t 196 0.428 ± 0.012 0.266 ± 0.12 0.22 ± 0.036a
h4 399 -(monoplex) N N
Mouse actin 238 - - -
  1. IOD of PCR product bands is a mean of three observations, quantified with Gel Pro 3.1 image analysis software.
  2. Details of primers given in Table 3.
  3. aSignificant difference.
  4. N, No differences seen in monoplex RTPCR.