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Table 1 Expression of chromatin condensing genes in FD/CPA-treated rat testis

From: Putative molecular mechanism underlying sperm chromatin remodelling is regulated by reproductive hormones

Rat genes

PCR product (bp)

Ratio

(Mean ± S.D.) = IOD of specific gene/ β Actin

Control

FD

CPA

ube1)

363

0.271 ± 0.03

0.124 ± 0.06

0.079 ± 0.02a

ube2d2

379

1.261 ± 0.016

1.324 ± 0.052

1.564 ± 0.07a

huwe1(e3)

365

0.41 ± 0.087

0.35 ± 0.04

0.33 ± 0.16

hdac6

417

0.13 ± 0.003

0.108 ± 0.01

0.061 ± 0.001a

ubB

238

-(monoplex)

N

N

cdyl

344

0.824 ± 0.02

0.246 ± 0.009a

0.602 ± 0.002a

brdt

382

1.826 ± 0.03

1.43 ± 0.018a

1.40 ± 0.02a

hdac1

162

0.668 ± 0.062

0.53 ± 0.13

0.41 ± 0.05a

miwi/piwi

400

1.115 ± 0.003

0.92 ± 0.25

0.925 ± 0.02a

mbd2

357

0.13 ± 0.01

0.109 ± 0.007

0.073 ± 0.01a

pem

187

0.0154 ± 0.0006

0.02 ± 0.003

0.0015 ± 0.0003a

cremΔC-G

322

-(monoplex)

N

N

h2a somatic

320

0.131 ± 0.01

0.17 ± 0.0009

0.05 ± 0.005a

h2b somatic

113

0.094 ± 0.005

0.099 ± 0.003

0.059 ± 0.007a

h3 somatic

345

0.549 ± 0.07

0.37 ± 0.03

0.47 ± 0.18

th2a

320

0.872 ± 0.053

0.877 ± 0.06

0.712 ± 0.002

th2b

113

3.4426 ± 0.66

3.11 ± 0.73

3.25 ± 0.217

h3t

196

0.428 ± 0.012

0.266 ± 0.12

0.22 ± 0.036a

h4

399

-(monoplex)

N

N

Mouse actin

238

-

-

-

  1. IOD of PCR product bands is a mean of three observations, quantified with Gel Pro 3.1 image analysis software.
  2. Details of primers given in Table 3.
  3. aSignificant difference.
  4. N, No differences seen in monoplex RTPCR.