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Table 1 Yield and overall quality of whole genome amplification (WGA) products of bisulfite-modified DNA

From: A systematic evaluation of whole genome amplification of bisulfite-modified DNA

Sample WGA yield (ng) Number of the detected CpGa Average methylation signal Average unmethylation signal
no amplification 1 - 484,857 (99.81 %) 1,764.202 2,537.532
no amplification 2 - 484,841 (99.81 %) 1,967.142 2,682.317
no amplification 3 - 484,831 (99.81 %) 1,882.296 2,875.583
WGA 50 ng 1 4,400 480,869 (98.99 %) 1,262.738 2,584.268
WGA 50 ng 2 6,000 480,159 (98.85 %) 1,266.639 2,113.009
WGA 50 ng 3 4,680 479,465 (98.70 %) 1,354.389 2,347.615
WGA 10 ng 1 4,300 465,730 (95.88 %) 1,208.001 2,257.573
WGA 10 ng 2 6,260 463,572 (95.43 %) 1,082.716 2,190.567
WGA 10 ng 3 6,020 467,036 (96.14 %) 1,292.537 1,931.764
DDW1 68 - - -
DDW2 58 - - -
DDW3 14 - - -
  1. aDetection P value below 0.01 considered significant detection.
  2. WGA, whole genome amplification; DDW, deionized distilled water.