Table 1 Yield and overall quality of whole genome amplification (WGA) products of bisulfite-modified DNA
From: A systematic evaluation of whole genome amplification of bisulfite-modified DNA
Sample | WGA yield (ng) | Number of the detected CpGa | Average methylation signal | Average unmethylation signal |
|---|---|---|---|---|
no amplification 1 | - | 484,857 (99.81 %) | 1,764.202 | 2,537.532 |
no amplification 2 | - | 484,841 (99.81 %) | 1,967.142 | 2,682.317 |
no amplification 3 | - | 484,831 (99.81 %) | 1,882.296 | 2,875.583 |
WGA 50 ng 1 | 4,400 | 480,869 (98.99 %) | 1,262.738 | 2,584.268 |
WGA 50 ng 2 | 6,000 | 480,159 (98.85 %) | 1,266.639 | 2,113.009 |
WGA 50 ng 3 | 4,680 | 479,465 (98.70 %) | 1,354.389 | 2,347.615 |
WGA 10 ng 1 | 4,300 | 465,730 (95.88 %) | 1,208.001 | 2,257.573 |
WGA 10 ng 2 | 6,260 | 463,572 (95.43 %) | 1,082.716 | 2,190.567 |
WGA 10 ng 3 | 6,020 | 467,036 (96.14 %) | 1,292.537 | 1,931.764 |
DDW1 | 68 | - | - | - |
DDW2 | 58 | - | - | - |
DDW3 | 14 | - | - | - |