Figure 3

Boxplots of validation and replication JIA case-control data for differentially methylated genes MRPL28 and IL32 . (A) HM27 MRPL28 array data (probe cg12437481) for the discovery case-control pairs, and MRPL28 sequenom data (assay unit CpG_14.15 – see Additional file1: Figure S3) for the discovery and replication case-control pairs. (B) HM27 IL32 array data (probe cg18350391) for the discovery case-control pairs, and IL32 sequenom data (assay unit CpG_10 – see Additional File 1: Figure S4) for the discovery and replication case-control pairs. Comparing array and sequenom data for the discovery samples demonstrates validation of array data, and comparing sequenom data between the discovery and replication samples demonstrates replication of the direction of effect and magnitude of difference between the cases and controls at these loci