Vitamin and antioxidant rich diet increases MLH1 promoter DNA methylation in DMT2 subjects

Clinical Epigenetics

Table 2 MLH1 region 1 group-specific methylation levels for each CpG, mean methylation over the seven CpGs, and paired t -test of methylation changes from baseline to eight weeks

Health group		CpG 1	CpG 3	CpG 4	CpG 5	CpG 6	CpG 7	CpG 8	Mean
NIDDM2		0,666*	0,195	0,810*	−0,025	1,759*	0,032	1,660	0,728*
	SE	0,250	0,429	0,295	0,388	0,539	0,320	1,086	0,254
	P	0,026	0,661	0,023	0,950	0,010	0,924	0,161	0,019
IFG		1,250	0,919	1,202	0,466	0,547	2,028	1,372	1,112*
	SE	0,502	0,502	0,740	0,424	0,786	1,828	0,898	0,278
	P	0,068	0,141	0,180	0,333	0,525	0,330	0,201	0,016
LC		−0,138	−0,060	−0,165	0,173	0,498	−0,064	0,401	0,092
	SE	0,180	0,322	0,190	0,251	0,227	0,186	0,285	0,184
	P	0,487	0,861	0,433	0,529	0,093	0,748	0,232	0,642

CpG numbers 1, 4, 6, and the mean methylation were significantly higher methylated in the IDDM2 group. The IFG group had significantly higher overall methylation. Methylation level remained stable in the LC group. Asterisks indicate significances at P < 0.05. NIDDM2, patients with non-insulin-dependent diabetes mellitus type 2; IFG, patients with impaired fasting glucose; LC, lean control group; SE, standard error of the mean.

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