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Table 5 Foxp3 pyrosequencing/primer design

From: Secondhand smoke in combination with ambient air pollution exposure is associated with increasedx CpG methylation and decreased expression of IFN-γ in T effector cells and Foxp3 in T regulatory cells in children

 

Foxp3 promoter

Foxp3 intronic region

Forward

5′-CAAATTCAGAGTATTAGTTCTTTTCCTCTTT−3′

5′-TTGGGTTAAGTTTGTTGTAGGAT−3′

Reverse

5′-[Biotin]-GTGGCATGTCGCACCAAAAAGAAGAG−3′

5′-[Biotin]- ACCCCCCACTTACCCAAATTTT−3′

Sequencing

5′-AAAGGCAAATTCA-3′

5′-GTAGGATAGGGTAGTTAG-3′

Description

6 CpG sites*

7 CpG sites*

 

(-141, -95, -80, -66, -54, -27)

(+3951, +3956, +4105, +4224, +4228, +4236, +4245)

  1. *CpG sites indicate nucleotide position in relation to transcription start. Symbols – and + indicate upstream and downstream of transcription start, respectively. CpG, cytosine nucleotide neighboring guanine.