IGF2/H19 hypomethylation is tissue, cell, and CpG site dependent and not correlated with body asymmetry in adolescents with Silver-Russell syndrome

Table 5 Basal proliferation of skin fibroblast cultures

Number of seeded cells	OD_d4/OD_d1^a			OD_d7/OD_d4^b
	S1 to S5	K1 to K3	T- test	S1 to S5	K1 to K3	T- test
	Mean (n = 16)^c	Mean (n = 10)^d	P	Mean (n = 16)^c	Mean (n = 10)^d	P
200 cells/well	4.25 ± 2.04	5.33 ± 1.67	0.17	2.41 ± 1.33	2.09 ± 0.74	0.49
1600 cells/well	2.92 ± 1.22	3.54 ± 1.01	0.19	1.36 ± 0.26	1.33 ± 0.40	0.81

^aMean absorption from (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2 H-tetrazolium, inner salt (MTS) test after 4 days in culture divided by that after 1 day in culture; ^bmean absorption from MTS test after 7 days in culture divided by that after 4 days in culture; ^ctwo tests done for each of the cultures from patients S1, S2, S3, and S5 L/R; ^dtwo tests done for each of the cultures from K1L, K2, and K3 L/R. S, patient with Silver-Russell syndrome; K, control patient; L, left side; R, right side.

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