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Table 3 M1 to M5 methylation levels of clonal fibroblast cultures from Silver-Russell syndrome (SRS) patient S2 and control patient K1

From: IGF2/H19 hypomethylation is tissue, cell, and CpG site dependent and not correlated with body asymmetry in adolescents with Silver-Russell syndrome

Fibroblast culture

Origin/clone

na

% Methylation of individual MS-MLPA sites

   

M1

M2

M3

M4

M5

S2L (short side)

origin

2

4b ± 3

13 ± 8

17 ± 6

62 ± 10

97 ± 6

1

2

26 ± 8

2 ± 1

45 ± 5

47 ± 1

114 ± 7

2

2

66 ± 10

1 ± 1

58 ± 7

66 ± 2

118 ± 5

3

2

1 ± 1

0 ± 0

6 ± 1

43 ± 5

90 ± 4

4

2

34 ± 3

0 ± 0

49 ± 7

44 ± 8

100 ± 3

S2R (long side)

origin

2

1 ± 1

3 ± 0

15 ± 6

31 ± 5

100 ± 1

1

2

0 ± 0

0 ± 0

5 ± 2

0 ± 0

99 ± 13

2

2

1 ± 1

0 ± 0

10 ± 2

44 ± 1

107 ± 4

3

2

0 ± 0

0 ± 0

1 ± 1

2 ± 2

84 ± 13

4

2

0 ± 0

4 ± 4

11 ± 1

6 ± 1

78 ± 7

K1L

origin

2

17 ± 7

35 ± 8

43 ± 11

70 ± 6

92 ± 21

1

3

0 ± 0

44 ± 3

59 ± 8

44 ± 7

120 ± 10

2

3

0 ± 0

51 ± 6

51 ± 5

75 ± 10

99 ± 8

3

2

1 ± 1

33 ± 10

44 ± 5

49 ± 1

99 ± 10

4

2

0 ± 0

44 ± 2

43 ± 5

60 ± 7

82 ± 5

5

3

38 ± 2

45 ± 6

49 ± 1

43 ± 9

100 ± 19

6

3

1 ± 0

28 ± 6

61 ± 7

81 ± 7

94 ± 9

7

2

1 ± 1

26 ± 1

39 ± 2

102 ± 4

91 ± 3

  1. aNumber of MS-MLPA determinations; bMS-MLPA determined methylation level in%. MS-MLPA, methylation-specific multiple ligation-dependent probe amplification; L, left; R, right.