Figure 3
IGF2 and H19 expression in skin fibroblast cultures. IGF2 and H19 mRNA levels were determined by real time (RT)-PCR. See Additional file 6 for data in tabular form. (A) IGF2 and H19 expression values from the individual cultures are related. Virtually no correlation was present for the control cultures (rPearson = −0.12) and a positive correlation for the Silver-Russell syndrome (SRS) cultures (rPearson = 0.96). (B) The IGF2 expression values are related to the imprinting center region 1 (ICR1) methylation value (mean of MS-MLPA sites M1 to M5) from the same culture (Table 2). (C) The IGF2 expression values are related to the methylation values from site M2 only (see Additional file 4 for tabular methylation data).