Figure 3

Representative JAK2 mutation analysis of MPN patient samples by allele-specific PCR. The 203 base pairs (bp) product is specific for the mutant allele, whereas the 364 bp product amplifying both mutant and wild-type alleles serves as an internal PCR control. DNA from a healthy donor (#1287) was used as a negative control. DNA from a patient with homozygous JAK2V617F mutation served as a positive control (#1222, mutation verified by sequencing). In a dilution series of DNA from a patient with a homozygous JAK2V617F mutation with normal DNA, the sensitivity of the PCR could be shown to be at least 1/64. M = DNA ladder. Patient samples #1537, #1541, #1542, #1543, and #1546 carry a JAK2V617F mutation.