Figure 3

The effect of ribavirin alone or in combination with TSA or SAHA on ESR1 mRNA expression in MDA-MB-231 cells as measured by real-time PCR. Cells were treated with 900 μM ribavirin for 72 h alone or followed by 12 h treatment with 10 μM SAHA or 0.33 μM TSA (n = 3). Every experiment has shown the same effect as to the expression of ESR1 - however with a significant variation among the single experiments. A representative experiment is shown.