Table 5 Primer sequences and annealing temperature.
| Genes | Sequences |
Annealing temperature |
|---|---|---|
| APC M |
F:GAACCAAAACGCTCCCCAT R:TTATATGTCGGTTACGTGCGTTTATAT | 61 |
| APC U |
F:AAACCAAAACACTCCCCATTC R:AGTTATATGTTGGTTATGTGTGTTTAT | 56 |
| ATM M |
F:GGAGTTCGAGTCGAAGGG R:CTACCTACTCCCGCTTCCGA | 65 |
| ATM U |
F:GTTTTGGAGTTTGAGTTGAAGGGT R:AACTACCTACTCCCACTTCCAA | 60 |
| GAPDH (Real Time-PCR) |
F: CCACTCCTCCACCTTTGAC R: ACCCTGTTGCTGTAGCCA | 60 |
| APC(Real Time-PCR) | F:CTTCAAAATTACCTCCAC R:CTCCTCTAACTCCTTCTC | 60 |
| ATM(Real Time-PCR) | F:CAGGGTAGTTTAGTTGAG GTTGACAG R:CTATACTGGTGGTCAGTGCCAAAGT | 60 |