Table 5 Primer sequences and annealing temperature.
Genes | Sequences | Annealing temperature |
|---|---|---|
APC M | F:GAACCAAAACGCTCCCCAT R:TTATATGTCGGTTACGTGCGTTTATAT | 61 |
APC U | F:AAACCAAAACACTCCCCATTC R:AGTTATATGTTGGTTATGTGTGTTTAT | 56 |
ATM M | F:GGAGTTCGAGTCGAAGGG R:CTACCTACTCCCGCTTCCGA | 65 |
ATM U | F:GTTTTGGAGTTTGAGTTGAAGGGT R:AACTACCTACTCCCACTTCCAA | 60 |
GAPDH (Real Time-PCR) | F: CCACTCCTCCACCTTTGAC R: ACCCTGTTGCTGTAGCCA | 60 |
APC(Real Time-PCR) | F:CTTCAAAATTACCTCCAC R:CTCCTCTAACTCCTTCTC | 60 |
ATM(Real Time-PCR) | F:CAGGGTAGTTTAGTTGAG GTTGACAG R:CTATACTGGTGGTCAGTGCCAAAGT | 60 |