Figure 5
From: Promoter de-methylation of cyclin D2 by sulforaphane in prostate cancer cells
Sulforaphane increased transcript levels of cyclin D2. LnCap cells were treated with SFN (15 and 30 μM) for 24 h. RNA was extracted and cDNA synthesized for qRT-PCR. SFN increased transcript levels of cyclin D2 compared with vehicle (DMSO). Values are fold change of gene copy numbers normalized to GAPDH and compared to vehicle, and represent means ± SEM, n = 3. P values represent treatment values compared to vehicle.