Sulforaphane decreased promoter methylation of cyclin D2. Methylation-specific PCR was performed after bisulfite modification of DNA from LnCap cells treated for 48 h. Upper figures are representative bands. UM indicates unmethylated cyclin D2 PCR products. M indicates methylated PCR products. The lower figure shows the levels of unmethylated versus methylated cyclin D2 PCR products by densitometry based on biological triplicates. SFN and 5-Aza-dC both decreased cyclin D2 promoter methylation. P values represent treatment values compared to vehicle.