Sulforaphane decreased global DNA methylation. LnCap cells were treated with SFN (15 and 30 μM) or 5-Aza-dC (5 μM) for 24 h. Genomic DNA was extracted and treated with sodium bisulfite. Global methylation status was fluorometrically quantified in a microplate-based ELISA assay. Values were derived as fold change compared to vehicle (DMSO) and represent means ± SEM, n = 3. P values represent treatment values compared to vehicle.