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Figure 2 | Clinical Epigenetics

Figure 2

From: Promoter de-methylation of cyclin D2 by sulforaphane in prostate cancer cells

Figure 2

Sulforaphane decreased global DNA methylation. LnCap cells were treated with SFN (15 and 30 μM) or 5-Aza-dC (5 μM) for 24 h. Genomic DNA was extracted and treated with sodium bisulfite. Global methylation status was fluorometrically quantified in a microplate-based ELISA assay. Values were derived as fold change compared to vehicle (DMSO) and represent means ± SEM, n = 3. P values represent treatment values compared to vehicle.

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