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Fig. 2 | Clinical Epigenetics

Fig. 2

From: Identification of ChIP-seq mapped targets of HP1β due to bombesin/GRP receptor activation

Fig. 2

Genomic PCR for genes identified by microarray and ChIP-seq as regulated by GRPR signaling via HP1β. Genomic DNA was extracted from both wild-type Caco-2 cells (+) and cells that had been exposed to GRPR siRNA for 72 h (−) and immunoprecipitated after exposure to antibodies directed against HP1β, H3K9, or IGG. DNA was then subject to PCR with primers targeting genes determined to be both expressed differentially during microarray analysis and displaying a ChIP Sequencing Peak in the local area

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