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Table 4 Global DNA methylation in tissues from ApoE mice fed a control (C) or high fat (HF) diet depleted of folic acid (−F) or folic acid and vitamins B6 and B12 (−F−B) for 16 weeks

From: Differential effects of nutritional folic acid deficiency and moderate hyperhomocysteinemia on aortic plaque formation and genome-wide DNA methylation in vascular tissue from ApoE-/- mice

Global DNA methylation

Control fat

High fat

C

C−F

C−F−B

HF

HF−F

HF−F−B

Aorta

5-methyldeoxycytidine (ng/μg DNA)

125.5 ± 8.2 (6)

135.7 ± 7.7 (6)

134.4 ± 22. (6)

116.6 ± 11.5 (6)

123.7 ± 7.8 (6)

133.2 ± 15.7 (6)

DNA methylation (%)

3.72 ± 0.06 (6)

3.61 ± 0.05 (6)

3.62 ± 0.07 (6)

3.60 ± 0.05 (6)

3.57 ± 0.04 (6)

3.58 ± 0.07 (6)

Heart

5-methyldeoxycytidine (ng/μg DNA)

158.9 ± 10.9 (6)

135.3 ± 4.8 (6)

130.8 ± 3.7 (6)

134.7 ± 6.1 (6)

157.0 ± 13.4 (6)

148.8 ± 9.8 (6)

DNA methylation (%)

3.8 ± 0.08 (6)

3.68 ± 0.11 (6)

3.75 ± 0.04 (6)

3.79 ± 0.05 (6)

3.85 ± 0.12 (6)

3.81 ± 0.05 (6)

Aorta tunica adventitia

5-methyldeoxycytidine (ng/μg DNA)

137.9 ± 12.2 (9)

141.0 ± 8.6 (10)

184.9 ± 22.3 (10)

171.5 ± 13.0 (8)

149.7 ± 20.1 (9)

157.8 ± 16.2 (7)

DNA methylation (%)

4.05 ± 0.11 (10)

3.94 ± 0.15 (10)

4.25 ± 0.10 (10)

4.31 ± 0.12 (8)

3.99 ± 0.10 (9)

3.98 ± 0.13 (7)

Liver

5-methyldeoxycytidine (ng/μg DNA)

68.1 ± 5.5 (10)

66.6 ± 4.7 (10)

71.4 ± 5.3 (10)

71.5 ± 5.9 (10)

68.4 ± 6.5 (10)

67.6 ± 6.6 (10)

DNA methylation (%)

3.19 ± 0.04 (10)

3.20 ± 0.04 (10)

3.21 ± 0.05 (10)

3.21 ± 0.05 (10)

3.20 ± 0.06 (10)

3.21 ± 0.05 (10)

  1. Values are means ± SEM with the number of mice sampled per group in parentheses