Fig. 2
Changes in respiration kinetics in relation to SIRT3 overexpression. a SIRT3 overexpression starts to increase respiration approximately 12 h after transfection with a maximum increase by 30–35% around 21 h after transfection (baseline = controls; presented increase of respiration of cells transfected with SIRT3 was the result of the percent rise of SIRT3 transfected cells after distraction of the percent rise of the controls (cells transfected with the inactive SIRT3 mutant and versus untransfected cells). b 20 h post transfection the metabolic rates of cells transfected with SIRT3 wt or controls transfected with the SIRT3H248Y inactive mutant or untransfected cells were compared (N = 3 independent experiments/7 independent measurements). c Western blot signals of overexpressed mitochondrial SIRT3 (mSIRT3-Flag) via Flag antibody detection shows first signals 6 h post transfection and a signal maximum intensity 16 to 20 h after transfection. An increase of SIRT3 protein expression concurs with an increase in respiration, which was shown in two independent experiments (four independent measurements)